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Thursday, December 29, 2011
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Monday, December 5, 2011
How Pipettes Have Advanced Liquid Handling
The most used piece of laboratory equipment, in any laboratory setting, is the pipette. This useful and extremely important tool is used in nearly every area of scientific, medical, biotech, pharmaceutical, and chemistry laboratory around the world today. From the simple plastic transfer pipette to the ultra-precise automated pipetting system, laboratory pipettes have found an important niche in the scientific disciplines.
The transfer pipette is one of the most used pipettes in any scientific industry. These pipettes are manufactures in variable sizes, and can be graduated for rough measurement of liquids. They are usually made of polyurethane, and have a flexible plastic bulb for drawing up liquids. You can purchase sterile, individually wrapped pipettes for many uses.
Laboratory pipettes will always be in use in every laboratory where fast or precision measurement and delivery of fluids is needed. Even simple disposable transfer pipettes are being graduated, for precise transfer of fluids, and for quick measurement of analytes and reagents. This is effective for the fast-moving laboratory, and can be useful for STAT procedures as well. Transfer pipette come in many volumes, from 1 ml up to 20 ml transfer syringes. Purchases of large amounts of these pipettes lend convenience to the large volume laboratory.
Graduated glass pipettes are needed for the precision pipetting of fluids for reagent setup or culture mixing. These precision pipettes can be vacuum assisted to eliminate mouth aspiration. Glass graduated pipettes can be sterilized via autoclave, without concern for variations in volume due to high temperatures, by the use of high-annealed glass products such as Pyrex. Plastic graduated pipettes are a one-time use solution for the laboratory on the go. These useful pipettes can be used once and disposed of; creating an environment that reduces cross-contamination of specific cultures or reagents.
Precision pipette systems have been used for decades, but now the precise nature of pipette systems today are computer chip controlled, lending to a much more reliable method of fluid transfer. These newer systems give such precision that most biotech and pharmaceutical disciplines need them. Disposable plastic pipette tips are used in conjunction of handheld pipetting devices that utilize a piston-type aspiration and delivery system. There are also autopipette systems that use diaphragmatic aspiration and delivery via computer control, giving the digital age a foothold on the market.
Bench top pipette systems for the precision delivery of reagents and chemicals into test tubes or cuvettes are in use at a high level, as they give convenience for the laboratory personnel. This method can be used for large volumes of chemicals that get large usage during shifts, and most of these pipetting systems can be easily refilled. Other pipetting systems are available for large volumes, such as those needed for mass chemistry analysis procedures.
Robotic pipette systems are used by many biotech laboratories for the automation of many processes that would otherwise take up personnel time. This increases productivity, and can result in mass testing procedures. These automated robotic systems can be set up on conveyor systems for easier delivery of chemicals and fluids.
The pipette systems of the future will not need much improvement, since the pipettes used today satisfy the world's laboratories needs.
Thursday, November 24, 2011
Carol Ann Duffy: New Selected Poems
L.P. Hartley once wrote 'the past is a foreign country; they do things differently there.' Carol Ann Duffy explores the relative 'foreignness' of recollection revealing both its reassuring familiarity and its unexpected revelation. This conflict between voluntary and involuntary memory; between what we think we know and what we find we didn't dare to know or admit, forms the 'foreign' land of much of Carol Ann Duffy's poetic landscape. I say landscape deliberately. Duffy's evocation of the past conjures up worlds and words very much concerned with territory and 'ownership' and in this poem 'Litany' we see such how the resurrection of the past represents who we are, and what we are and were.
Duffy loves lists. Indeed lists are a way that Duffy can ironise our relation to the past. Such lists inspire collusion and a spirited humorous collusion at that. Every time I read a Duffy list I admire the very developed degree of selectivity and peculiar attentiveness employed by the poet to make such a list work; to make it representative of the message and era she has elected to represent and re-animate.
When we read the first stanza of 'Litany' those of us who can recall the 1960s smilingly tick off the resonances and connotatations of Duffy's acknowledged world. It feels so right, so present to us. This 'presence' is then used as the basis for the more 'inside' revelation. The poet uncovers the secret tensions behind half-understood childhoods through the play between recognition and misrecognition.
Duffy deploys a simile: 'sly like a rumour' to risk a revelation. For Duffy's childhood recollection is now narrated by an adult and adults may convert half-glimpsed fascination into definitive knowledge. This tension between a the writer who is an adult and the writer who was the child under renders Duffy's revisitation of the past both comical and tragic.
For this territory is a world where words were infantilised for the sake of politeness, for the sake of social sanitisation and stability. Coffee mornings and 'get togethers' skirted around authenticity and truth.Children were expected to know nothing. But Duffy knows how curious children are about the unsaids, about the secret worlds and words of the adults; of family friends.
Duffy rediscovers the superficiality of social connection, and ironises it heavily. How lonely was such a childhood we wonder? How lonely indeed for the adults trying to conform and to present themselves as relentlessly normal? Safe,'normal' words imprisoned and suffocated relationships. We wonder of course how far things have actually changed?
Duffy makes us retouch the signs of the past. Thinking arrives through sensory recollection. We experience a past that we may or may not have directly experienced through resonant sensory detail and this makes us involved. We are seduced by the pride in pyrex and the grand 'lounge' of the past!
We remember cellophane. We hear its name once again. 'Polyester' has become transmutated into a joke; a failed symbol of pragmatic enterprise( one does not have to iron it) with erotic nullity. ( It produces static and is distinctly sweatyand erotically unappetising!) The juxtaposition of the different senses makes the reader extend their involvement within this world of the 'Lounge' and the suppressed word; memory is truly resurrecting..and uncomfortable!
It is a world of conventional relationships and behaviours. Anything that could undermine such a world is feared and abjected:
'An embarrassing word, broken to bits..'
Duffy's astute alignment of biscuit and unlooked for testimony is throwaway and yet devastating. Protocol twitches at the mention of something real, unsightly and unmentionable.
Sex and death intervene in the memory of the child and destabilise the rigid boundedness of such a 'reality' so that the transgression instigated by the looming knowledge of sex, reedits the past. The litany of names in the final stanza operates as much as an ironic obituary now for Duffy's narrator as for background detail and verfication. These names are now most like absences, they are 'hauntings' and only survive through the humanity and humour of Duffy's excavation into the words upon which we rest (somewhat anxiously perhaps) the past.
I can still hear the coffee cups!
Monday, November 21, 2011
Cuisinart Chef's Classic Nonstick Hard-Anodized 10-Piece Cookware Set with Easy Grip Riveted Silicone Handles
!±8± Cuisinart Chef's Classic Nonstick Hard-Anodized 10-Piece Cookware Set with Easy Grip Riveted Silicone Handles
Inspired by the great French kitchens, Cuisinart began making professional cookware over 30 years ago. Constructed of the finest materials available, to perfectly perform all of the classic cooking techniques. Designed to last a lifetime, Cuisinart cookware makes family meals memorable and entertaining a pleasure. Savor the Good Life right at home, with family and friends. Cuisinart makes it absolutely delicious.Designed from the inside out for today's lifestyles, our Non-Stick Hard Anodized Cookware combines uncompromising superior cooking performance with a professional look. Cuisinart Non-Stick Hard Anodized Cookware exterior is harder than stainless steel for durability and professional performance. We guarantee a lifetime of healthy cooking with Cuisinart Chef's Classic Non-Stick Hard Anodized Cookware.Chef's Classic Hard Anodized 10pc. Set: More options for more meals! Two skillets, two saucepans, a sauté pan and stockpot let you prepare a variety of meals in a variety of ways.
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Sunday, November 13, 2011
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Wednesday, November 9, 2011
Chicken Soup With Chinese Herbs Recipe
Chicken soup has been used as a folk remedy for respiratory illnesses for a long time. In 2000, the scientific exploration of this claim began and has continued. Wikipedia reports, "Chicken soup has long been touted as a form of folk medicine to treat symptoms of the common cold and related conditions. In 2000, scientists at the University of Nebraska Medical Center in Omaha studied the effect of chicken soup on the inflammatory response in vitro. They found that some components of the chicken soup inhibit neutrophil migration, which may have an anti-inflammatory effect that could hypothetically lead to temporary ease from symptoms of illness.[1] However, since these results have been obtained from purified cells (and directly applied), the diluted soup in vivo effect is debatable. The New York Times reviewed the University of Nebraska study, among others, in 2007 and concluded that "none of the research is conclusive, and it's not known whether the changes measured in the laboratory really have a meaningful effect on people with cold symptoms."[2]."1
Chicken soup is one of my favorite things to cook. The recipe below is meant to make a large pot of soup. It is a living recipe in the sense that the basic format stays the same but I will often rotate ingredients in or out of it. When I feel like more green vegetables I am likely to add bok choi or zuccini. Sometimes I add fingerling or yukon-gold potatoes. I often make a wild rice blend separately and add it to the finished product. Part of the fun of cooking this soup for me is the experimental factor.
One important way that I vary the recipe is by which Chinese herbs I choose to include in the soup. The varieties have to do with the ends that I am trying to achieve with the formula. It takes some skill to achieve this without making the soup inedible due to the strong flavors of Chinese herbs. Here are some simple herbal formulas you can experiment with:
Immune-boost: Huang Qi (astragalus root) 30g, Fang Feng (ledebouriella root) 10g, Bai Zhu (atractylodis macrocephelae) 10g (don't use when you are already sick!)
Qi-boost: Huang Qi (astragalus root) 30g, Dang Shen (codonopsis root) 30g, Bai Zhu (atractylodis macrocephelae) 10g (don't use when you are already sick!)
Blood-Builder: Gou Qi Zi (Goji berry, Chinese wolfberry) 30g, Hong Zao (jujube date) 15 pieces, Long Yan Rou (longan fruit) 15 pieces, Dang Gui Tou (head of Chinese angelica root) 1 piece
Lung Yin Vacuity (dry cough): Bai He (lilly bulb) 30g, Mai Men Dong (tuber ophiopogonis japonici) 15g, Jing Jie (Herba Seu Flos Schizonepetae Tenuifoliae) 15g
Insomnia: Suan Zao Ren (zizyphus seed) 15g, Wu Wei Zi (schizzandra berry) 15g, He Huan Pi (mimosa tree bark) 10g, Bai Zi Ren (biota seed) 10g
These are just some examples... there are many more possibilities! You will need to spend some time finding a reputable source of good quality Chinese herbs to purchase and use them in your soups. On to the recipe, below.
1 5-6 lb whole chicken
2 large yellow onions, chopped
2-3 large leeks, halved and sliced thickly
2 bunches green onions, chopped
2-3 large shallots, chopped
1 head of garlic, sliced thickly
1 bunch celery, chopped
8-10 medium carrots, chopped
6 medium parsnips, chopped
3 to 4-inch piece of fresh ginger, 1/2 sliced thickly with skin, 1/2 peeled & julienned
2 Tbs Herbs De Provence, crushed in mortar with pistil
Chinese herbs (as above), washed & soaked
2 cartons Organic Chicken Stock
1.5 lbs Shiitake mushrooms
1/4 cup Tamari Sauce
3 Tbs Mirin
Phase I
Wash and soak Chinese herbal formula for 30 minutes. Wash and chop and/or peel one onion & one shallot. Chop half the garlic, half the celery, half the carrots, half the parsnips and half the ginger. Remove the Shiitake stems and chop up the stems (they are used for the stock). Remove the root part of the leeks and wash. Remove the root and white parts of the green onions, wash them and chop them. Add all of these vegetables to a large soup pot (at least 6 qt sized or bigger). Remove the giblets from the chicken. Thoroughly rinse the chicken under cold running water. Place the chicken in the pot on top of the stock vegetables & Chinese herbs. Add water to cover the chicken or up to 1 inch below the top of the pot. Place the pot on high heat, cover and bring to a boil. Remove lid and turn down heat to medium-low (enough to maintain a mild boil). Boil for 30-40 minutes until the internal temperature of the chicken has reached 165 degrees F (as measured with a meat thermometer). While it's boiling, use a large spoon to skim the surface of the grayish foam that accumulates. Carefully remove the chicken from the pot and place on a cutting board, let cool. Turn down the stove to low and allow the stock to simmer. Remove skin from the chicken and discard. Remove the meat from the bones and carcass and place in a Pyrex container with a sealing lid for refrigerator storage. Crack the bones and carcass and place back into the simmering stock. Simmer for an additional 40-60 minutes.
Phase II
While the stock is simmering, wash, peel and/or chop and/or slice the remaining vegetables. Place the Shiitakes and the green onions in one bowl and the rest of the vegetables in another larger bowl, set aside. Take to pot off the heat and strain the stock. Discard the dregs of the stock vegetables, herbs, bones & carcass. Add vegetables & Herbs De Provence to the pot and pour the stock over them. Add Mirin and chicken stock (from containers) to bring volume back up to the top of the pot. Place back on the burner and bring to a boil. Lower heat to medium-low and boil for about 25 minutes. Remove the pot from the heat, uncovered. Add mushrooms, green onions, and Tamari and serve. Before refrigerating, let the soup cool down for at least 30 minutes. For each serving, add chicken meat from the separate container to taste.
Enjoy!
Sunday, November 6, 2011
World Kitchen 1058994 Pyrex Bakeware 19-Piece Baking Dish Set, Clear
!±8±World Kitchen 1058994 Pyrex Bakeware 19-Piece Baking Dish Set, Clear
Brand : World KitchenRate :

Price : $59.95
Post Date : Nov 06, 2011 23:12:05
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Pyrex Bakeware 19-piece set includes 1-each 2-quart oblong dish, 1-each 3-quart oblong dish, 1-each 8-inch square dish, 1-each 1-1/2-quart loaf dish, 1-each 1-quart mix bowl, 1-each 1-quart mix bowl dark blue plastic cover, 1-each 1-1/2-quart mix bow, 1-e